CST, 95666S, beta-crystallin B1/crybb1 Rabbit Antibody

Polyclonal Antibody for studying CRYBB1 mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, beta-crystallin B1/crybb1 Rabbit Antibody (CST #95666) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity beta-crystallin B1/crybb1 Rabbit Antibody recognizes endogenous levels of total β-crystallin B1 protein. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu244 of mouse β-crystallin B1 protein. Antibodies are purified by peptide affinity chromatography. Background β-crystallin B1, encoded by , is a member of the β-crystallins, one of three major crystallin types encoded by the mammalian genome, which also includes alpha- and gamma-crystallins. Crystallins are expressed at very high levels in the retinal lens and form homo/heterodimers via its N-terminal arms (1). Crystallins generally function to maintain the transparency and refractive power of the eye lens. However, they are expressed outside of the lens in other tissues and have alternative non-lens functions (2). For example, Nuc1 mutant rat, in which the β gene is mutated, exhibit vascular remodeling abnormalities via an astrocyte-dependent mechanism (3,4). The precise cellular function of β-crystallins in glia is not well understood but may involve regulation of endolysosomal acidification, Golgi trafficking, and specific forms of cell death (5,6). Interestingly, is highly enriched in microglia outside of the mammalian lens and may serve as a distinct marker for microglia (7). Alternate Names 3110006K12Rik; BB1CRY; Beta-B1 crystallin; Beta-crystallin B1; Beta-crystallin B1B; CRBB1; Crybb1; crystallin, beta B1; OTTMUSP00000029911; OTTMUSP00000029913 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 28 SOURCE: Rabbit