CST, 9568T, NHERF2 (D3A5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NHERF2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NHERF2 (D3A5) Rabbit Monoclonal Antibody (CST #9568) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NHERF2 (D3A5) Rabbit Monoclonal Antibody recognizes endogenous levels of total NHERF2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NHERF2 protein. Background Na /H exchanger regulatory factor 2 (NHERF2) is one of four proteins in the NHERF protein family. It is closely related to NHERF1 and, like NHERF1, contains two PDZ domains and a C-terminal ezrin/radixin/moesin (ERM) binding domain (EBD). Along with the other members of this protein family, NHERF2 is abundantly present in the mammalian small intestine and colon where it plays a central role in trafficking, membrane retention, dimerization, and regulation of ion channels and membrane proteins (1). NHERF2 is a scaffolding protein that recruits membrane proteins to the apical membrane by tethering them to the apical cytoskeleton via its ERM domain (2). It has been shown that the NHERF proteins bind to the Na /H exchanger 3 (NHE3) in the brush border of intestinal epithelial cells. NHE3 accounts for the majority of neutral NaCl absorbtion and NHERF proteins play an essential role in NHE3 regulation (3). Alternate Names E3KARP; MGC104639; Na(+)/H(+) exchange regulatory cofactor NHE-RF2; NHE3 kinase A regulatory protein E3KARP; NHE3 regulatory factor 2; NHE3RF2; NHERF-2; NHERF2; NHRF2; OCTS2; SIP-1; SIP1; SLC9A3 regulator 2; SLC9A3R2; Sodium-hydrogen exchanger regulatory factor 2; sodium/hydrogen exchanger 3 kinase A regulatory protein; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 2; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2; Solute carrier family 9 isoform A3 regulatory factor 2; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 2; SRY-interacting protein 1; TKA-1; Tyrosine kinase activator protein 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 46 Source/Isotype: Rabbit IgG