CST, 95746T, Rab7 (E9O7E) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Rab7. Validated for WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Rab7 (E9O7E) Mouse Monoclonal Antibody (CST #95746) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Rab7 (E9O7E) Mouse Monoclonal Antibody recognizes endogenous levels of total Rab7 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu188 of human Rab7 protein. Background Rab7 and Rab9 are members of the Ras superfamily of small Rab GTPases (1). Both proteins are located in late endosomes, but exert different functions. Rab7 associates with the RIPL effector protein to control membrane trafficking from early to late endosomes and to lysosomes (2,3). Rab7 also helps to regulate growth receptor endocytic trafficking and degradation (3,4), and maturation of phagosome and autophagic vacuoles (4-6). Rab9 interacts with its effector proteins p40 and TIP47 (7,8) to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network (9,10). Alternate Names CMT2B; FLJ20819; PRO2706; RAB7; RAB7, member RAS oncogene family; RAB7A; RAB7A, member RAS oncogene family; Ras-associated protein RAB7; Ras-related protein Rab-7a Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 23 Source/Isotype: Mouse IgG1