CST, 9578S, Cleaved Drosophila Dcp-1 (Asp215) Antibody

Polyclonal Antibody for studying Dcp-1 (Asp215) cleaved Drosophila. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Cleaved Drosophila Dcp-1 (Asp215) Antibody (CST #9578) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Cleaved Drosophila Dcp-1 (Asp215) Antibody recognizes endogenous levels of the large 22 kDa fragment of cleaved Dcp-1. This antibody does not recognize full length Dcp-1. This antibody also detects a non-specific, apoptotic-related band at 50 kDa by western blot. Species Reactivity: D. melanogaster Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp215 of Drosophila Dcp-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Cell death in the fruit fly Drosophila melanogaster is regulated by many of the same stimuli as mammalian cell death (1). The Drosophila genome contains seven caspase genes; three encode initiator caspases, and four encode effector caspases (reviewed in (2)). The Drosophila effector caspase, death caspase-1 (Dcp-1), is a critical executioner of apoptosis. It is involved in the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). The activation of Dcp-1 requires proteolytic processing of its inactive zymogen into active p22 and p13 fragments (3). Comparison of the in vivo activity between DrICE and Dcp-1 has shown that DrICE is a more effective inducer of apoptosis than Dcp-1, which instead plays a role in determining the rate of cell death (4). Alternate Names CASP7 iso2; Caspase-1; Caspase-1 subunit p13; Caspase-1 subunit p22; CG5370; Dcp-1; Dcp1 Specification REACTIVITY: Dm SENSITIVITY: Endogenous MW (kDa): 22 SOURCE: Rabbit