CST, 95848S, GADS (E4O1V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GADS. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, GADS (E4O1V) Rabbit Monoclonal Antibody (CST #95848) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GADS (E4O1V) Rabbit Monoclonal Antibody recognizes endogenous levels of total GADS protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly172 of human GADS protein. Background GRB2-related adaptor downstream of Shc (GADS) belongs to the GRB2 family of adaptor proteins. It is a hematopoietic cell-specific signaling adaptor protein that harbors amino- and carboxy-terminal SH3 domains, a central SH2 domain, and a unique linker region that is rich in proline and glutamine residues (1). The presence of SH2 and SH3 domains within GADS strongly suggest that it functions in signal transduction cascades by facilitating protein-protein interactions. In the context of T cells, research studies have demonstrated that GADS interacts with LAT and SLP-76 signaling complexes to facilitate NFAT activation downstream of TCR engagement (2-4). Given its role as a fundamental mediator of TCR signaling, GADS is subject to multiple modes of negative regulation to limit TCR signal strength. For example, research studies have demonstrated that HPK1 directly phosphorylates GADS at Thr262 within its linker region, a modification that promotes 14-3-3 binding and dissociation of signaling complexes nucleated by LAT, SLP-76, and GADS (5). The linker region of GADS is also subject to caspase-mediated cleavage, which separates its SH2 and SH3 domains and thus impairs the ability of GADS to bridge LAT and SLP-76 signaling complexes for transduction of faithful TCR signals (6,7). Alternate Names Adapter protein GRID; GADS; GRAP-2; GRAP2; GRB-2-like protein; GRB2 related adaptor protein 2; GRB2-related adapter protein 2; GRB2-related adaptor protein 2; GRB2-related protein with insert domain; GRB2L; GRBLG; GRBX; Grf-40; Grf40; Grf40 adapter protein; GRID; Growth factor receptor-binding protein; growth factor receptor-bound protein 2-related adaptor protein 2; GRPL; Hematopoietic cell-associated adapter protein GrpL; hematopoietic cell-associated adaptor protein GRPL; Mona; P38; Protein GADS; SH3-SH2-SH3 adapter Mona; SH3-SH2-SH3 adaptor molecule Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG