CST, 9585S, Slug (C19G7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Slug. Validated for WB,IP,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, Slug (C19G7) Rabbit Monoclonal Antibody (CST #9585) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 80121 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Slug (C19G7) Rabbit Monoclonal Antibody detects endogenous levels of total Slug protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant human Slug protein. The epitope has been mapped to residues surrounding Tyr32. Background Slug (SNAI2) is a widely expressed transcriptional repressor and member of the Snail family of zinc finger transcription factors (1). Similar to the related Snail protein, Slug binds to the E-cadherin promoter region to repress transcription during development (2). The binding of Slug to integrin promoter sequences represses integrin expression and results in reduced cell adhesion (3). Down regulation of E-cadherin expression occurs during the epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (4,5). The tumor suppressor protein p53 induces Slug expression in γ-irradiated cells; Slug protects damaged cells from apoptosis by repressing p53-induced transcription of the proapoptotic Bcl-2 family protein Puma (6). Deletion mutations in the corresponding Slug gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism, while a genetic duplication resulting in Slug overexpression is associated with a collection of congenital heart defects termed tetralogy of Fallot (7). Alternate Names MGC10182; Neural crest transcription factor Slug; Protein snail homolog 2; SLUG; slug (chicken homolog), zinc finger protein; SLUGH; SLUGH1; SNAI2; snail 2; snail family transcriptional repressor 2; snail family zinc finger 2; snail homolog 2; snail homolog 2 (Drosophila); Snail2; WS2D; Zinc finger protein SNAI2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG