CST, 95895S, HDGF2 (Cryptic Specific) Antibody

Polyclonal Antibody for studying HDGF2. Validated for Western Blotting. Highly specific and rigorously validated in-house, HDGF2 (Cryptic Specific) Antibody (CST #95895) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HDGF2 (Cryptic Specific) Antibody recognizes endogenous levels of total HDGF2 cryptic protein. This antibody does not detect wild-type HDGF2 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys260 of human HDGF2 cryptic protein. Background Hepatoma-derived growth factor-related protein 2 (HDGF2), encoded by the gene , is a ubiquitously expressed histone-binding protein belonging to the larger family of hepatoma-derived growth factor (HDGF) and HDGF-related proteins (HRPs). Structurally, HDGF2 and its family members share a highly conserved amino terminus region, termed homologous to the amino terminus of HDGF (HATH). Contained within the HATH region is a Pro-Trp-Trp-Pro (PWWP) domain, which recognizes and binds histone H3 lysine 36 di- and trimethylation. HDGF2 functions through this domain to regulate chromatin structure and DNA repair (1-3). Exon splicing of HDGF2 mRNA is regulated by TAR DNA-binding protein 43 (TDP43), a ubiquitously expressed DNA/RNA-binding protein belonging to the heterogeneous nuclear ribonucleoprotein (hnRNP) family. Dysfunctional TDP43, driven by genetic mutations, hyperphosphorylation, ubiquitination, and cleavage forms insoluble cytoplasmic aggregates. These aggregates are pathological hallmarks of patients with amyotrophic lateral sclerosis (ALS), frontotemporal lobar degeneration (FTLD), and limbic predominant age-related TDP-43 encephalopathy (LATE). These inclusions are also present in some patients with other neurodegenerative diseases (NDDs), including Alzheimer's disease (AD) and Chronic traumatic encephalopathy (CTE) (4,5). TDP43 cytoplasmic mislocalization and aggregation lead to altered HDGF2 mRNA splicing and the inclusion of cryptic exons. This results in an alternative translated HDGF2 protein that includes cryptic exon-encoded neo epitopes. Neo epitope-containing HDGF2 is detected in CSF and plasma, suggesting it may be a biomarker for neurodegenerative diseases with TDP43 pathology (6,7). Alternate Names HDGF like 2; HDGF-2; HDGF-related protein 2; HDGF2; HDGFL2; HDGFRP2; HDGR2; Hepatoma-derived growth factor 2; Hepatoma-derived growth factor-related protein 2; HRP-2; HRP2; MGC2641 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 135 SOURCE: Rabbit