CST, 95901S, GITR (E9O9H) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying GITR mouse. Validated for Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, GITR (E9O9H) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #95901) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Flow Cytometry (Live): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity GITR (E9O9H) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val95 of mouse GITR protein. Background TNFRSF18, also known as glucocorticoid-induced tumor necrosis factor-receptor (TNFR)-related protein (GITR) and activation-inducible TNFR family receptor, encodes a type 1 membrane protein of the TNF-receptor superfamily (1). Three alternatively spliced transcript variants encoding distinct isoforms have been reported (2). GITR is an immune cell co-stimulatory receptor expressed constitutively at high levels on CD4 CD25 T regulatory cells (Tregs), at low levels on naïve and memory T cells, and is induced upon T cell activation (3-5). Studies show GITR can also be induced on NK cells, macrophages, and DCs (3,4,6). Although GITR does not have intrinsic enzymatic activity, TNFSF18 (also known as GITRL) expressed on antigen presenting cells binds to GITR, resulting in recruitment of TNFR-associated factor family members and activation of the NF-κB pathway in T cells (7). GITR ligation has been shown to play a role in CD8 T cell activation, cytotoxicity, and memory T cell survival (8-10). In the thymus, GITR is thought to play a key role in dominant immunological self-tolerance through thymic Treg differentiation and expansion (11). Of note, GITR ligation inhibits Treg suppressive function (12-13) and promotes effector T cell resistance to Treg suppression (14-15). Due to the combined effects on both Treg suppression and effector cell activation, GITR represents a unique opportunity for immunotherapeutic intervention in cancer (16). Alternate Names AITR; CD357; Gitr; glucocorticoid-induced TNFR-related protein; Tnfrsf18; TNR18; Tumor necrosis factor receptor superfamily member 18; tumor necrosis factor receptor superfamily, member 18 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG