CST, 95938SF, TCF12/HEB (E5R8X) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TCF12/HEB. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, TCF12/HEB (E5R8X) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #95938) is ready to ship. Product Usage Information This product is the carrier free version of product #78245. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 78245 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TCF12/HEB (E5R8X) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total TCF12/HEB protein. Non-specific staining was observed in smooth and skeletal muscle by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to residues surrounding Asn450 of human TCF12/HEB protein. Background TCF12/HEB (T cell factor 12/HeLa E-box binding protein) is a member of the E proteins that are type I basic helix-loop-helix domain-containing transcription factors that bind directly to the E-box DNA consensus sequence and control numerous developmental processes (1,2). E protein family members include E2A, E2-2, and TCF12/HEB. TCF12/HEB forms homo- or heterodimers with other E proteins to regulate transcription of target genes that play a critical role in the development of T cells, B cells, and plasmacytoid dendritic cells (2-4). The gene has two transcriptional start sites leading to the generation of the long form called HEBCan, and the short form, HEBAlt (5). The two isoforms are thought to have distinct functions, with HEBCan playing a role throughout T cell development, while HEBAlt functions in the efficient generation of T cell precursors (5). Alternate Names bHLHb20; class B basic helix-loop-helix protein 20; CRS3; DNA-binding protein HTF4; E-box-binding protein; HEB; helix-loop-helix transcription factor 4; Helix-loop-helix transcription factor 4 isoform c; HsT17266; HTF4; p64; TCF-12; TCF12; Transcription factor 12; Transcription factor 12 (HTF4, helix-loop-helix transcription factors 4), isoform CRA_b; transcription factor HTF-4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 85 Source/Isotype: Rabbit IgG