CST, 95994S, HAND1 (E7C4L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HAND1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, HAND1 (E7C4L) Rabbit Monoclonal Antibody (CST #95994) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HAND1 (E7C4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total HAND1 protein. This antibody may detect nonspecific bands at 140 and 200 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly79 of human HAND1 protein. Background HAND1 is a basic helix-loop-helix (bHLH) transcription factor (TF) that is capable of forming heterodimers with other binding partners, functioning as a transcriptional activator or a repressor depending on the underlying sequence and dimerization partner (1,2). HAND1 is a key regulator of trophoblast giant cell differentiation and cardiac morphogenesis (3). Moreover, several studies have demonstrated that this TF plays an essential role in cell proliferation and carcinogenesis. Namely, HAND1 has been reported to be downregulated in several cancers, including pancreatic, colorectal, and small cell lung cancers (4-7). Furthermore, gene expression is negatively regulated by the high mobility group A1 (HMGA1) protein in human thyroid carcinomas (8) and its expression was shown to be necessary in sustaining gastrointestinal stromal tumor (GIST) proliferation (9). Alternate Names BHLHA27; Class A basic helix-loop-helix protein 27; EHAND; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1; HAND1; heart and neural crest derivatives expressed 1; Heart- and neural crest derivatives-expressed protein 1; Hxt; Thing1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 24 Source/Isotype: Rabbit IgG