CST, 96053T, IFITM2 (F2H3I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IFITM2 mouse. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, IFITM2 (F2H3I) Rabbit Monoclonal Antibody (CST #96053) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IFITM2 (F2H3I) Rabbit Monoclonal Antibody recognizes endogenous levels of total IFITM2 protein. This antibody cross-reacts with overexpressed mouse IFITM3 protein by western blot. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu20 of mouse IFITM2 protein. Background Interferon-induced transmembrane protein (IFITM) family members are composed of short amino- and carboxy-termini, two transmembrane domains, and a cytoplasmic domain (1). There are four family members in humans: IFITM1, IFITM2, IFITM3, and IFITM5 (2,3). Mice have two additional family members, IFITM6 and IFITM7 (2,3). Basal expression of IFITM proteins is observed in some cells and expression can also be induced by type I and type II interferons (4-6). The primary function of IFITM family proteins appears to be viral restriction, as IFITM proteins inhibit cytosolic entry of viruses by preventing fusion of viral and host membranes (7,8). The mechanism by which IFITM proteins inhibit fusion is unclear. Although IFITM proteins are present on both the plasma membrane and intracellular membranes, they most effectively restrict viral fusion in late endosomes and lysosomes (8,9). In addition, different family members exhibit specific viral preferences (9). For example, IFITM3 is most effective at restricting influenza A infection, while IFITM1 is more successful in controlling filoviruses and SARS (9,10). Alternate Names Dispanin subfamily A member 2c; DSPA2c; fra; Fragilis protein 3; Fragilis R; fragilis3; Ifit; Ifitm2; Ifitm2 protein; Ifitm3l; IFM2; Interferon induced transmembrane protein 2; interferon induced transmembrane protein 3-like; interferon induced transmembrane protein, like; Interferon-induced transmembrane protein 2; MCG22584; MGC102396; mil; mil-3 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 16-20 Source/Isotype: Rabbit IgG