CST, 9606S, Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation,Peptide ELISA (DELFIA). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse Monoclonal Antibody (CST #9606) is ready to ship. Product Usage Information Western Blotting: 1:4000 Immunoprecipitation: 1:20 Peptide ELISA (DELFIA): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse Monoclonal Antibody binds peptides and proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. By ELISA, this antibody recognizes a wide range of peptides containing this phosphorylated 14-3-3 binding motif in a manner that is phospho-specific and largely independent of the surrounding amino acid sequence. This antibody weakly cross-reacts with sequences containing phospho-Thr instead of phospho-Ser in this motif, and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No cross-reactivity is observed with corresponding nonphosphorylated sequences or with other phospho-Ser/Thr/Tyr containing motifs. This antibody complements our polyclonal Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601 by showing slightly different and overlapping specificity. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with phospho-(Ser) 14-3-3 binding motif peptides . Background The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (1-5). Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions (6). Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins (7). Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25C, FKHRL1, PKC and c-Raf (5,7). Phospho-(Ser) 14-3-3 Binding Motif Polyclonal and (4E2) Monoclonal Antibodies provide powerful tools for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery. Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1