CST, 96098S, GATA-3 (E2N1Y) Mouse Monoclonal Antibody

Monoclonal Antibody for studying GATA3. Validated for WB,IHC,IF,F,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, GATA-3 (E2N1Y) Mouse Monoclonal Antibody (CST #96098) is ready to ship. Product Usage Information The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:400 Immunofluorescence (Immunocytochemistry): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:100 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 57847 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), CUT&RUN Specificity / Sensitivity GATA-3 (E2N1Y) Mouse Monoclonal Antibody recognizes endogenous levels of total GATA-3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GATA-3 protein. Background GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA-binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). GATA-3 is a critical regulator of development of various systems in both mouse and human (4). GATA-3 mouse embryos die between E11 and E12 due to growth retardation and deformities in the brain and spinal cord (5). The function of GATA-3 has been extensively studied in T cell development and has recently been shown to be a downstream target of Notch in Notch-mediated differentiation of TH2 cells (6,7). It is expressed in both hematopoietic and non-hematopoietic tissues, including the kidney, skin, mammary gland, and central nervous system (8-10). Decreased expression of GATA-3 in luminal breast cancer is associated with poor clinical outcome. GATA-3 expression level may therefore be a promising prognostic biomarker (11). Haploinsufficiency of GATA-3 results in Barakat syndome in humans, a condition characterized by sensorineural deafness and renal dysplasia (12). Alternate Names GATA binding protein 3; GATA-binding factor 3; GATA3; HDR; HDRS; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Mouse IgG1