CST, 96099T, c-IAP1 (F7C3U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying c-IAP1. Validated for WB,WB,IP,F. Available in 2 sizes. Highly specific and rigorously validated in-house, c-IAP1 (F7C3U) Rabbit Monoclonal Antibody (CST #96099) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:200 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity c-IAP1 (F7C3U) Rabbit Monoclonal Antibody recognizes endogenous levels of total c-IAP1 protein. This antibody does not cross-react with c-IAP2 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human c-IAP1 protein. Background The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2). c-IAP1 (gene name: ), which is structurally and functionally closely related to c-IAP2 (gene name: ), also contains a RING domain that confers E3 ubiquitin ligase activity (7). c-IAP1 is recruited to the TNF receptor complex upon ligand binding and functions as a positive regulator of the canonical NF-κB signaling pathway, leading to autoubiquitination as well as ubiquitination of other signaling targets, including RIP1 and TRAF family members (8-11). c-IAP is frequently overexpressed in many cancers, contributing to tumorigenesis by inhibiting apoptosis (12,13). IAP inhibitors, like Smac mimetics, can result in c-IAP1 degradation and have been pursued as therapeutic opportunities (14). Alternate Names API1; apoptosis inhibitor 1; baculoviral IAP repeat containing 2; baculoviral IAP repeat-containing 2; Baculoviral IAP repeat-containing protein 2; BIRC2; C-IAP1; Cellular inhibitor of apoptosis 1; cIAP1; hIAP-2; hIAP2; IAP homolog B; IAP-2; IAP2; Inhibitor of apoptosis protein 2; MIHB; NFR2-TRAF signalling complex protein; RING finger protein 48; RING-type E3 ubiquitin transferase BIRC2; RNF48; TNFR2-TRAF-signaling complex protein 2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG