CST, 96167T, TRIM37 (D7U2L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRIM37. Validated for Western Blotting,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, TRIM37 (D7U2L) Rabbit Monoclonal Antibody (CST #96167) is ready to ship. Product Usage Information Western Blotting: 1:1000 Flow Cytometry (Fixed/Permeabilized): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity TRIM37 (D7U2L) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRIM37 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His511 of human TRIM37 protein. Background Tripartite motif containing protein 37 (TRIM37, also known as MUL1) is a member of the tripartite motif (TRIM) family whose members contain a RING domain, a B-box, and a coiled-coil region (together called RBCC). TRIM37 was initially described as the defining truncation mutation in mulibrey nanism (1). An E3 ubiquitin ligase, TRIM37 is responsible for the monoubiquitination of histone H2A at Lys119, and is amplified in over 40% of breast cancers (2,3). TRIM37 interacts with polycomb group proteins EZH2 and SUZ12, and contributes to histone H3K27 lysine tri-methylation and subsequent silencing of tumor suppressor genes (3). Oncogenic activity of TRIM37 has also been reported in pancreatic, hepatic, and colorectal cancers (4-7). Alternate Names E3 ubiquitin-protein ligase TRIM37; KIAA0898; MUL; Mulibrey nanism protein; POB1; RING-B-box-coiled-coil protein; RING-type E3 ubiquitin transferase TRIM37; TEF3; TRI37; TRIM37; tripartite motif containing 37; tripartite motif-containing 37; Tripartite motif-containing protein 37 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 135 Source/Isotype: Rabbit IgG