CST, 9621L, Phospho-(Ser/Thr) PKA Substrate Antibody

Polyclonal Antibody for studying . Validated for WB,IP,IHC,E. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-(Ser/Thr) PKA Substrate Antibody (CST #9621) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 - 1:200 Peptide ELISA (DELFIA): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Phospho-(Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine residue with arginine at the -3 position. This antibody is a useful tool in identifying substrates of AGC family kinases, including PKA and PKC. This antibody does not cross-react with the nonphosphorylated PKA substrate motif. Species Reactivity: All Species Expected Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-PKA substrate peptide. Antibodies are purified by protein A and peptide affinity chromatography. Background An important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery. Specification REACTIVITY: All SENSITIVITY: Endogenous SOURCE: Rabbit