CST, 96227S, EZHIP Antibody

Polyclonal Antibody for studying EZHIP. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, EZHIP Antibody (CST #96227) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity EZHIP Antibody recognizes endogenous levels of total EZHIP protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala495 of human EZHIP protein. Antibodies are purified by peptide affinity chromatography. Background EZH inhibitory protein (EZHIP), also called CXorf67 or Catacomb, is a protein discovered in aggressive posterior fossa A ependymomas (1-3). EZHIP contains a highly conserved sequence surrounding a methionine near its C-terminus which mimics the activity of the oncohistone protein H3K27M, and allows it to bind EZH2 and inhibit PRC2 function to lower H3K27me2/3 levels (2-4). EZHIP has also been shown to play a regulatory role in developing sperm and oocytes (5). Alternate Names CATACOMB; catalytic antagonist of Polycomb; CXorf67; EZH inhibitory protein; EZHIP; K27M-like inhibitor of PRC2; KIP75; MGC47837; uncharacterized protein CXorf67 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 SOURCE: Rabbit