CST, 96248SF, PTPRF/LAR (E6W4X) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying LAR. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, PTPRF/LAR (E6W4X) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #96248) is ready to ship. Product Usage Information This product is the carrier free version of product #61611. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 61611 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity PTPRF/LAR (E6W4X) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total PTPRF/LAR protein. This antibody detects the E-subunit of the processed PTPRF/LAR protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein from a central region of the human PTPRF/LAR protein. Background Receptor type protein tyrosine phosphatase F (PTPRF, LAR) is a transmembrane PTP that helps to regulate insulin signaling, cell proliferation and cell migration. The PTPRF protein is composed of an extracellular segment that contains several Ig-like and fibronectin (Fn-III) domains, a transmembrane region and a pair of cytoplasmic phosphatase domains (1,2). Functional studies reveal that the membrane-associated D1 phosphatase domain is responsible for substrate dephosphorylation, while the D2 domain is important for substrate specificity (3). PTPRF negatively regulates insulin signaling through dephosphorylation of insulin receptor and insulin receptor substrate (4). This phosphatase activates the pro-apoptotic DAPK serine/threonine kinase by removing a phosphate at Tyr491/492, while the kinase Src replaces the phosphate to inactivate DAPK at the same time it down regulates PTPRF expression (5). PTPRF is commonly found at focal adhesions where it interacts with liprin, which localizes the phosphatase to the membrane, and the Rac/Rho family GTPase Trio (6). Localization of PTPRF at adherens junctions results in PTPRF modification of β-catenin, which inhibits cell migration by limiting the amount of available cytosolic β-catenin (7). Alternate Names BNAH2; FLJ43335; FLJ45062; FLJ45567; LAR; LCA-homolog; leukocyte antigen-related (LAR) PTP receptor; leukocyte antigen-related tyrosine phosphatase; Leukocyte common antigen related; protein tyrosine phosphatase receptor type F; protein tyrosine phosphatase, receptor type, F; protein tyrosine phosphatase, receptor type, F polypeptide; PTPRF; receptor-linked protein-tyrosine phosphatase LAR; Receptor-type tyrosine-protein phosphatase F Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 150, 213 Source/Isotype: Rabbit IgG