CST, 9624T, Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation,Peptide ELISA (DELFIA). Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit Monoclonal Antibody (CST #9624) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Peptide ELISA (DELFIA): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit Monoclonal Antibody detects peptides and proteins containing a phospho-Ser/Thr residue with arginine at the -3 and -2 positions. This antibody is a useful tool in identifying new substrates of PKA. This antibody recognizes other -3 arginine-bearing phospho-Ser/Thr peptides, such as substrate motifs for Akt and PKC, to a lesser extent. This antibody does not recognize the nonphosphorylated substrate motif peptides. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with synthetic phospho-PKA substrate peptides . Background An important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery. Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG