CST, 9625T, Rab6 (D37C7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RAB6. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Rab6 (D37C7) Rabbit Monoclonal Antibody (CST #9625) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63722 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Rab6 (D37C7) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rab6 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human Rab6 protein. Background Rab6 is a member of the Ras superfamily of small Rab GTPases implicated in endocytosis (1). The three distinct members of the Rab6 subfamily (Rab6A, Rab6A', and Rab6B) are structurally similar but likely exhibit non-overlapping functions (2,3). Rab6 localized to the Golgi (4) regulates retrograde transport of membrane-bound target proteins from the Golgi apparatus to the endoplasmic reticulum (5-7) or from the Golgi to the endosome during exocytotic transport (8). Rab6 interacts with microtubule motor proteins such as rabkinesin-6 (KIF20A) and dynein/dynactin complexes; Rab6-mediated transport requires a functionally intact microtubule system (9,10). Rab6 also regulates cytokinesis and cell cycle check point through interactions with Rab6 effector proteins, including the dynein/dynactin protein DCTN1 and the GTPase activating protein RABGAP1 (11,12). Alternate Names GTP-binding protein RAB6A'; GTP-binding protein RAB6B; Oncogene RAB6; Rab GTPase; Rab-6; RAB6; RAB6, member RAS oncogene family; RAB6A; Rab6A variant 3; RAB6A, member RAS oncogene family; RAB6A'; RAB6B; Ras-related protein Rab-6A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 24 Source/Isotype: Rabbit