CST, 96305S, LC3B (E5Q2K) Mouse Monoclonal Antibody (InTraSeq™ 3' Conjugate 3062)

Monoclonal Antibody for studying LC3B. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, LC3B (E5Q2K) Mouse Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3062) (CST #96305) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity LC3B (E5Q2K) Mouse Monoclonal Antibody (InTraSeq™ 3' Conjugate 3062) recognizes endogenous levels of total LC3B protein. This antibody conjugate detects both type I and type II forms of LC3B. Cross-reactivity was not detected with other family members. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LC3B protein. Background Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11). Alternate Names ATG8F; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3B; MAP1 light chain 3-like protein 2; MAP1A/1BLC3; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1ALC3; MAP1LC3B; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3B; MLP3B Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Mouse IgG2b kappa