CST, 96351S, CENP-E (E5N7B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CENPE. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CENP-E (E5N7B) Rabbit Monoclonal Antibody (CST #96351) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CENP-E (E5N7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total CENP-E protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro333 of human CENP-E protein. Background Centromere-associated protein E (CENP-E) is a kinesin-like motor protein and mitotic-checkpoint kinase BUB1B binding partner that is essential for establishing and maintaining stable attachments between mitotic chromosomes and spindle microtubules (1). CENP-E plays an important role as a motor protein in the alignment of chromosomes during prometaphase (2). Research studies indicate that CENP-E protein expression peaks in late G2 and M-phases of the cell cycle before the protein is degraded at mitotic exit (3). Additional studies show that the loss of CENP-E function results in cell cycle arrest in mitosis. Mutations in the corresponding gene can result in autosomal recessive primary microcephaly-13, a developmental disorder characterized by small head circumference, dysmorphic facial features, short stature, and delayed psychomotor development (4). Since CENP-E is essential for mitotic progression and is required for cellular proliferation, it has become an interesting target for cancer therapy (5-7). Alternate Names CENP-E; CENPE; Centromere autoantigen E (312kD); Centromere protein E; centromere protein E, 312kDa; Centromere-associated protein E; KIF10; kinesin family member 10; Kinesin-7; Kinesin-related protein CENPE; MCPH13; PPP1R61; protein phosphatase 1, regulatory subunit 61 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 270 Source/Isotype: Rabbit IgG