CST, 96396S, UFL1 (F5P6L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UFL1. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, UFL1 (F5P6L) Rabbit Monoclonal Antibody (CST #96396) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 18040 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity UFL1 (F5P6L) Rabbit Monoclonal Antibody recognizes endogenous levels of total UFL1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UFL1 protein. Background The ubiquitin-fold modifier 1 (UFM1) conjugation system (UFMylation) is a ubiquitin-like system that plays key roles in development and stress responses (1, reviewed in 2-4). Like ubiquitin, conjugation of UFM1 is a three-step enzymatic process involving a specific E1-activating enzyme (UBA5), an E2-conjugating enzyme (UFC1), and an E3-ligation enzyme (UFL1). Several substrates for UFMylation have been identified including DDRGK1, RPL26, p53, MRE11, histone H4, ASC1, CDK5RAP3, and CYB5R3 (5-12). Modification of these targets by UFM1 has direct effects on ER homeostasis, protein translation, and response to DNA damage. Notably, independent screens identified UFMylation as a key regulator of ER-phagy and autophagy (5,13,14). Aberrant UFMylation is associated with many pathological conditions, including cancer, diabetes, and inflammatory diseases (2-4). Alternate Names E3 UFM1-protein ligase 1; E3 UFM1-protein transferase 1; hypothetical protein LOC23376; KIAA0776; LZAP-binding protein; MAXER; Multiple alpha-helix protein located at ER; NLBP; novel LZAP-binding protein; RCAD; Regulator of C53/LZAP and DDRGK1; Regulator of CDK5RAP3 and DDRGK1; RP3-393D12.1; UFL1; UFM1 specific ligase 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG