CST, 96406S, DLL4 (D7N3H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DLL4. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, DLL4 (D7N3H) Rabbit Monoclonal Antibody (CST #96406) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity DLL4 (D7N3H) Rabbit Monoclonal Antibody recognizes endogenous levels of total DLL4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human DLL4 protein. Background Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3, and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus, where it activates transcription. Notch ligands may also be processed in a way similar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). DLL4 expression is highly restricted to the vascular endothelium (7), and haploinsufficiency of DLL4 results in major defects in vascular systems in mouse (8-11). Blockade of DLL4 inhibits tumor growth in model systems (12-14). Alternate Names AOS6; delta 4; delta ligand 4; delta like canonical Notch ligand 4; delta-like 4 (Drosophila); delta-like 4 homolog; delta-like 4 protein; Delta-like protein 4; Delta4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 75-80 Source/Isotype: Rabbit IgG