CST, 96407S, WDFY3 (F7P9J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Alfy. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, WDFY3 (F7P9J) Rabbit Monoclonal Antibody (CST #96407) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity WDFY3 (F7P9J) Rabbit Monoclonal Antibody recognizes endogenous levels of total WDFY3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human WDFY3 protein. Background WD repeat and FYVE domain-containing 3 (WDFY3), also known as Alfy, is a large scaffolding protein with multiple domains implicated in membrane trafficking, including the FYVE zinc-finger, WD repeats with conserved LC-interacting regions (LIR) motifs, and a Pleckstrin homology (PH)-Beige and Chediak-Higashi (BEACH) domain (1). WDFY3 regulates autophagy via interactions with phosphatidyl-inositol 3-phosphate, ubiquitin-binding autophagy receptors like p62/SQSTM1, and selective proteins in the Atg8 family, particularly GABARAPs (1-3). WDFY3 contributes to multiple forms of selective autophagy, such as mitophagy and aggrephagy (4). WDFY3 is highly expressed in the developing central nervous system, and mutations in human have been shown to be a risk factor for intellectual and developmental disabilities (5-7). Likewise, loss of in mice results in neurodevelopment defects (8,9). WDFY3 is also required for the clearance of aggregated mutant huntingtin protein to confer resistance to Huntington's disease (10). WDFY3 was also identified in a CRISPR screen as a regulator of efferocytosis, the phagocytic clearance of dying or dead cells (11). That study demonstrated that WDFY3 deficiency leads to impaired uptake of apoptotic cells and defects in degradation involving LC3-associated phagocytosis (LAP). Alternate Names AC095046.1; Alfy; Autophagy-linked FYVE protein; BCHS; KIAA0993; MCPH18; MGC16461; WD repeat and FYVE domain containing 3; WD repeat and FYVE domain-containing protein 3; WDFY3; ZFYVE25 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 395 Source/Isotype: Rabbit IgG