CST, 96420SF, KLF2 (E7K8Y) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying KLF2. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, KLF2 (E7K8Y) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #96420) is ready to ship. Product Usage Information This product is the carrier free version of product #51221. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 51221 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity KLF2 (E7K8Y) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total KLF2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human KLF2 protein. Background Krüppel-like factors (KLFs) are a family of zinc finger-containing DNA-binding transcriptional regulators that share homology with the Krüppel protein that regulates body segmentation. Seventeen mammalian KLFs have been identified so far and they regulate diverse biological processes, including development, differentiation, proliferation, growth, survival, and external stress responses. Alterations in their functions have been linked to many human diseases, including metabolic diseases and cancer (1-3). KLF2 is a key regulator in the differentiation and function of immune cells and vascular endothelial cells. KLF2 suppresses NF-κB-mediated gene expression and plays important roles in inflammatory response (4-7). Alternate Names KLF2; Krueppel-like factor 2; Kruppel like factor 2; Kruppel-like factor 2 (lung); Kruppel-like factor LKLF; LKLF; Lung krueppel-like factor; lung Kruppel-like factor; lung Kruppel-like zinc finger transcription factor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG