CST, 96422T, CPS1/Hep Par-1 (OCH1E5) Mouse Monoclonal Antibody (Clone previously known as Hepatocyte Specific Antigen)

Monoclonal Antibody for studying CPS1. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CPS1/Hep Par-1 (OCH1E5) Mouse Monoclonal Antibody (Clone previously known as Hepatocyte Specific Antigen) (CST #96422) is ready to ship. Product Usage Information IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in a PBS-based buffer containing sodium azide as a preservative. Stable for 12 months when stored at 4°C. Do not aliquot the antibody. Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity CPS1/Hep Par-1 (OCH1E5) Mouse Monoclonal Antibody (Clone previously known as Hepatocyte Specific Antigen) recognizes endogenous levels of total CPS1/Hep Par-1 protein, which is present in the liver and small intestine. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human liver fixed in formalin. Background Carbamoyl phosphate synthetase 1 (CPS1), a rate-limiting enzyme in the urea cycle, catalyzes the conversion of ammonia and bicarbonate to carbamoyl phosphate in mitochondria. Non-small cell lung carcinoma (NSCLC) cells with oncogenic and loss of the tumor suppressor LKB1 express CPS1 and depend on this enzyme for growth. CPS1 maintains pyrimidine/purine balance in these cancer cells. Silencing CPS1 reduces the pyrimidine to purine ratio and stalls DNA synthesis, leading to DNA damage and cancer cell death (1). In addition, the tumor suppressor p53 represses the expression of urea cycle enzymes CPS1, OTC, and ARG1 and, therefore, causes the accumulation of ammonia, suppressing cancer growth (2). Furthermore, research studies suggest that hypermethylation-mediated downregulation of expression may contribute to the progression of normal hepatocytes to hepatocellular carcinoma (HCC) (3). CPS1 was identified to be the antigen detected by Hep Par-1 antibody (4). Alternate Names carbamoyl-phosphate synthase (ammonia); Carbamoyl-phosphate synthase [ammonia], mitochondrial; carbamoyl-phosphate synthase 1; carbamoyl-phosphate synthase 1, mitochondrial; carbamoyl-phosphate synthetase 1, mitochondrial; Carbamoyl-phosphate synthetase I; carbamoylphosphate synthetase I; CPS1; CPSase I; CPSASE1; CPSM; PHN Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1