CST, 96428S, NR2F6/EAR-2 Antibody

Polyclonal Antibody for studying NR2F6. Validated for Western Blotting. Highly specific and rigorously validated in-house, NR2F6/EAR-2 Antibody (CST #96428) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NR2F6/EAR-2 Antibody recognizes endogenous levels of total NR2F6/EAR-2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly23 of human NR2F6/EAR-2 protein. Antibodies are purified by peptide affinity chromatography. Background NR2F6 (Nuclear receptor subfamily 2 group F member 6, EAR-2, COUP-TFIII) is a member of the NR2F family of transcription factors, defined by their role in the regulation of organogenesis, neurogenesis, and cellular differentiation during embryonic development (1,3). NR2F6 acts as a negative regulator for TRβ1-mediated transcriptional activity, a transcriptional repressor of IL-17 expression in Th-17 differentiated CD4 T cells, and a repressor of the NFAT/AP-1 complex on both the interleukin-2 and the interferon-γ cytokine promoters (2-5). NR2F6 has a non-redundant role in antagonizing anti-cancer effector T cell responses (5). It has been shown that loss of NR2F6 enhances the activity of established PD-L1 checkpoint blockade, promoting tumor regression and increased survival in mouse models (5,6). Alternate Names EAR-2; EAR2; ERBA-related gene-2; ERBAL2; NR2F6; Nuclear receptor subfamily 2 group F member 6; nuclear receptor subfamily 2, group F, member 6; nuclear receptor V-erbA-related; v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 2; V-erbA-related protein 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 43 SOURCE: Rabbit