CST, 9644L, 4E-BP1 (53H11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying 4E-BP1. Validated for WB,WB,IP,IHC,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, 4E-BP1 (53H11) Rabbit Monoclonal Antibody (CST #9644) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:1200 - 1:4800 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 42235 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity 4E-BP1 (53H11) Rabbit Monoclonal Antibody detects endogenous levels of total 4E-BP1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification 4E-BP1 (53H11) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1. Background Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). Alternate Names 4E-BP1; 4EBP1; BP-1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; MGC4316; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 15-20 Source/Isotype: Rabbit IgG