CST, 9647T, RUNX3/AML2 (D6E2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying AML2. Validated for WB,IP,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, RUNX3/AML2 (D6E2) Rabbit Monoclonal Antibody (CST #9647) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 86441 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity RUNX3/AML2 (D6E2) Rabbit Monoclonal Antibody recognizes endogenous levels of both isoforms of RUNX3 protein. This antibody recognizes mouse RUNX3/AML2 protein and is also reactive with human RUNX3/AML2; however, this antibody is not suggested for immunohistochemical analysis of human tissues. This antibody does not recognize RUNX1 and RUNX2 proteins. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RUNX3 protein. Background Runt-related transcription factor 3 (RUNX3, AML2), a member of the Runt family of transcription factors, plays an important role in the suppression of gastric epithelium cell proliferation (1), dorsal root ganglia neurogenesis (2), and T cell differentiation (3,4). RUNX3 is also involved in caspase-3-dependent apoptosis (5). Protein complexes containing RUNX3 and various transcription factors, such as Smads or β-catenin/TCF4, have tumor suppressor activity and regulate downstream target gene transcription (6,7). While typically localized to the nucleus, RUNX3 can be tyrosine phosphorylated and located in the cytoplasm of many cancer cells. This mislocalization of RUNX3 abolishes its tumor suppressor function and contributes to tumorigenesis (8). Research studies indicate that gene silencing or protein mislocalization inactivates RUNX3 in more than 80% of gastric cancers and other cancer types (1,9,10). Alternate Names Acute myeloid leukemia 2 protein; acute myeloid leukemia gene 2; AML2; CBF-alpha-3; CBFA3; Core-binding factor subunit alpha-3; core-binding factor, runt domain, alpha subunit 3; FLJ34510; MGC16070; Oncogene AML-2; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2-alpha C; PEBP2A3; PEBP2aC; Polyomavirus enhancer-binding protein 2 alpha C subunit; runt related transcription factor 3; Runt-related transcription factor 3; RUNX family transcription factor 3; RUNX3; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; transcription factor AML2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 43-48 Source/Isotype: Rabbit IgG