CST, 96610S, Spermine synthase (F9W1S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SMS. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Spermine synthase (F9W1S) Rabbit Monoclonal Antibody (CST #96610) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Spermine synthase (F9W1S) Rabbit Monoclonal Antibody recognizes endogenous levels of total spermine synthase protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human spermine synthase protein. Background Polyamines, including spermine, spermidine, and putrescine, are involved in many cellular processes, for example, cell proliferation, differentiation, apoptosis, the synthesis of proteins and nucleic acids, the regulation of ion channel activity, and the protection from oxidative injury (1). The cellular content of polyamines is tightly regulated; their biosynthesis is catalyzed by different enzymes, including spermine synthase (SMS) and spermidine synthase (SRM). The enzymes N1-acetylpolyamine oxidase (PAOX), spermidine/spermine N1-acetyltransferase (SAT1), and spermine oxidase (SMOX) are involved in the polyamine catabolism (1). SMS catalyzes spermine production from spermidine and decarboxylated S-adenosylmethionine (2). Mutations in cause Snyder-Robinson Syndrome, characterized by developmental delay, muscle/bone abnormalities, and intellectual disability (3-6). A recent study has indicated the potential role of polyamines in autophagy and tauopathy, where a reduction in SMS had a protective effect against tauopathy in a model and human cells (7). Alternate Names MRSR; SMS; Spermidine aminopropyltransferase; Spermine synthase; SPMSY; SpS; SPSY; SRS Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 41 Source/Isotype: Rabbit IgG