CST, 9663S, Caspase-3 Control Cell Extracts

Cell Extract Kit for studying Casp3 in the research area. Product Usage Information Boil for 3 minutes prior to use. Load 10 ul of untreated and cytochrome c treated Caspase-3 Control Cell Extracts per lane. Storage Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol,50 mM DTT, 0.01% w/v bromophenol blue or phenolred. Store at -20°C, or at -80°C for long-term storage. Background Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2). Alternate Names Apopain; casp3; casp367; casp39; caspase 3; caspase-3; caspase3; CPP 32; CPP-32; CPP32; Lysate; SCA-1; Yama Protein