CST, 96706S, Stat5 (D3N2B) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3114)

Monoclonal Antibody for studying STAT5A/STAT5B. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Stat5 (D3N2B) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3114) (CST #96706) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity Stat5 (D3N2B) Rabbit mAb (InTraSeq™ 3' Conjugate 3114) recognizes endogenous levels of total Stat5 protein. This antibody preferentially detects Stat5a, but may have weak reactivity for Stat5b. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Stat5 protein. Background Stat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6). Alternate Names epididymis secretory sperm binding protein; GHISID2; MGF; Signal transducer and activator of transcription 5A; Signal transducer and activator of transcription 5B; STA5A; STA5B; STAT5; STAT5A; STAT5B; transcription factor STAT5B Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG