CST, 9678S, Sec24A Antibody

Polyclonal Antibody for studying Sec24A. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Sec24A Antibody (CST #9678) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Sec24A Antibody detects endogenous levels of total Sec24A protein. This antibody does not cross-react with other members of Sec24 family. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe52 of human Sec24A protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Coat Protein Complex II (COPII) is composed of five cytosolic proteins: Sec23/24 complex, Sec13/31 complex, and Sar1. COPII coat is located at the ER/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23/24 complex, thereby forming a prebudding complex that directly binds target molecules (1-3). The prebudding complex further recruits Sec13/31 to form mature COPII coat (4,5). The Sec24 subunit of COPII coat is thought to play a critical role in cargo selection (2,6). It binds directly to cargo proteins at the ER and brings them to COPII vesicles through interaction with Sec23. There are four Sec24 isoforms in human cells: Sec24A, Sec24B, Sec24C, and Sec24D (7). In mice, mutations in Sec24B have been linked to developmental defects (8,9). Sec24bB has also been identified as a novel regulator of ferroptosis in microglia which can contribute to neurodegeneration (10). Alternate Names Protein transport protein Sec24A; SC24A; SEC24 family, member A; SEC24 family, member A (S. cerevisiae); SEC24 homolog A, COPII coat complex component; SEC24 related gene family, member A; SEC24-related protein A; SEC24A Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 120 SOURCE: Rabbit