CST, 96795S, SARS-CoV-2 Nsp2 (E3Q9Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying nsp2-pp1a-pp1ab SARS-CoV-2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SARS-CoV-2 Nsp2 (E3Q9Z) Rabbit Monoclonal Antibody (CST #96795) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SARS-CoV-2 Nsp2 (E3Q9Z) Rabbit Monoclonal Antibody recognizes infected levels of total SARS-CoV-2 Nsp2 protein. Species Reactivity: Virus Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of SARS-CoV-2 Nsp2 protein. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), nine accessory proteins, and four canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Nsp2 (non-structural protein 2) is derived from ORF1a and ORF1b (2). It is reported to interact with a protein involved in translational repression known as GIGYF2. GIGYF2 normally interacts with another translational repressor, 4EHP. These proteins together in a larger complex function to inhibit translation of mRNAs that may be defective during development. Upon infection with SARS-CoV-2, however, Nsp2 binds to GIGYF2 and exerts a stronger effect on host cell translation by 4EHP dependent and independent mechanisms, with inhibition of interferon translation being one of the notable effects (3). Alternate Names non-structural protein 2; non-structural protein 2 (181-818); nsp2; nsp2-pp1a-pp1ab; p65 homolog; SARS-CoV-2 nsp2 Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG