CST, 96825S, Fas (F7B6N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Fas mouse. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Fas (F7B6N) Rabbit Monoclonal Antibody (CST #96825) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Fas (F7B6N) Rabbit Monoclonal Antibody recognizes endogenous levels of total Fas protein. This antibody may detect a band of unknown origin around 70 kDa. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse Fas protein, within the intracellular region. Background Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers (1,2). Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes (3,4). FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-α, TRAIL, and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites (6). FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26 kDa trimeric soluble form (8,9). Alternate Names AI196731; Apo-1 antigen; APO1; Apoptosis-mediating surface antigen FAS; Apt1; CD95; Fas; Fas (TNF receptor superfamily member 6); Fas (TNF receptor superfamily member); Fas antigen; FASLG receptor; lpr; lymphoproliferation; TNF; Tnfr; TNFR6; Tnfrsf6; TNR6; Tumor necrosis factor receptor superfamily member 6 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 40-50 Source/Isotype: Rabbit IgG