CST, 96837S, VAMP3 (F1G2Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VAMP3 mouse. Validated for WB,WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, VAMP3 (F1G2Z) Rabbit Monoclonal Antibody (CST #96837) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:1200 Immunofluorescence (Immunocytochemistry): 1:1200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity VAMP3 (F1G2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total VAMP3 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse VAMP3 protein. Background Proteins in the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex are integral membrane proteins involved in vesicle transport and membrane fusion by pairing of vesicular SNAREs (v-SNAREs) with cognate target SNAREs (t-SNAREs) (reviewed in 1,2). Vesicle-associated membrane protein 3 (VAMP3), also known as cellubrevin, has a broad tissue distribution and localizes to endosomal compartments (3). VAMP3 interacts with the t-SNAREs syntaxin1, syntaxin4, SNAP23, and SNAP25 (4,5). Research studies indicate that VAMP3 is involved in transferrin receptor recycling to the plasma membrane (6) and T cell receptor recycling to immunological synapses (7). Inhibition of VAMP3 with tetanus toxin impairs membrane trafficking during cell migration (8). Alternate Names CEB; cellu; Cellubrevin; D130027G05Rik; OTTMUSP00000010900; RP23-455G15.3; Syb3; Synaptobrevin-3; VAM; VAMP-3; Vamp3; Vesicle-associated membrane protein 3 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 13 Source/Isotype: Rabbit IgG