CST, 9691S, Nitro-Tyrosine Antibody

Polyclonal Antibody for studying . Validated for Western Blotting. Highly specific and rigorously validated in-house, Nitro-Tyrosine Antibody (CST #9691) is ready to ship. Product Usage Information To obtain optimal results with this antibody please use PVDF instead of nitrocellulose membranes. Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Nitro-Tyrosine Antibody detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. This antibody is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples. This antibody does not cross-react with unmodified tyrosine or with phospho-tyrosine. Species Reactivity: All Species Expected Source / Purification Polyclonal antibodies are produced by immunizing animals with synthetic nitro-tyrosine-containing peptides . Antibodies are purified by protein A and peptide affinity chromatography. Background Nitric oxide (NO) is implicated in carcinogenesis (1), chronic infection, inflammation (2), and neurodegeneration (3). High levels of both superoxide and NO in tissues interact to form peroxynitrite, a potent oxidant that can modify Tyr residues in proteins to form 3-nitro-tyrosine (4). Tyrosine nitration of mitochondrial manganese superoxide dismutase results in loss of enzymatic activity (4). The nitration of p53 at Tyr residues abolishes its capacity for binding to its DNA consensus sequence (5). Specification REACTIVITY: All SENSITIVITY: Endogenous SOURCE: Rabbit