CST, 97038S, ATF-4 (E4Q4E) Mouse Monoclonal Antibody

Monoclonal Antibody for studying ATF-4. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, ATF-4 (E4Q4E) Mouse Monoclonal Antibody (CST #97038) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63435 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity ATF-4 (E4Q4E) Mouse Monoclonal Antibody recognizes endogenous levels of total ATF-4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro134 of human ATF-4 protein. Background ATF-4, an activating transcription factor/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway (1-3). ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4 (2). Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress (4). Alternate Names Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); ATF-4; ATF4; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2; CREB2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; Tax-responsive enhancer element-binding protein 67; TaxREB67; TXREB Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 49 Source/Isotype: Mouse IgG1 kappa