CST, 97099S, HEXIM1 (D5Y5K) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying HEXIM1. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, HEXIM1 (D5Y5K) Rabbit Monoclonal Antibody (PE Conjugate) (CST #97099) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity HEXIM1 (D5Y5K) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total HEXIM1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly63 of human HEXIM1 protein. Background Hexamethylene bis-acetamide-inducible protein 1 (HEXIM1) was originally identified in vascular smooth muscle cells as a protein that is upregulated upon treatment with the differentiating agent hexamethylene bisacetamide (1). HEXIM1 binds 7SK RNA, a highly abundant non-coding RNA, and together they act as a potent inhibitor of positive transcription elongation factor b (P-TEFb) (2,3). P-TEFb phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II and is an important regulator of transcription elongation (4-8). 7SK RNA-bound HEXIM1 interacts with the cyclin T1 subunit of P-TEFb, sequestering P-TEFb in an inactive form leading to transcription inhibition (2,3). The regulation of the relative ratio of inactive to active P-TEFb in the cell by HEXIM1/7SK RNA is thought to play a critical role in regulation of a wide range of cellular gene expression programs such as estrogen and glucocorticoid receptor regulated genes (9-12). Alternate Names Cardiac lineage protein 1; CLP1; EDG1; Estrogen down-regulated gene 1 protein; FLJ13562; hexamethylene bis-acetamide inducible 1; Hexamethylene bis-acetamide-inducible protein 1; hexamethylene bisacetamide inducible 1; hexamethylene bisacetamide-inducible protein; hexamethylene-bis-acetamide-inducible transcript 1; hexamthylene bis-acetamide inducible 1; HEXI1; HEXIM P-TEFb complex subunit 1; HEXIM1; HIS1; HMBA-inducible; MAQ1; menage a quatre 1; Menage a quatre protein 1; Protein HEXIM1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG