CST, 97109S, MKL1/MRTF-A (E2V2I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MKL1. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, MKL1/MRTF-A (E2V2I) Rabbit Monoclonal Antibody (CST #97109) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity MKL1/MRTF-A (E2V2I) Rabbit Monoclonal Antibody recognizes endogenous levels of total MKL1/MRTF-A protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly812 of human MKL1/MRTF-A protein. Background The megakaryoblastic leukemia proteins 1 and 2 (MKL1, MKL2) are myocardin-related transcription factors (MRTF-A, MRTF-B) that serve as actin-regulated transcription coactivators for the serum response factor (SRF). Interaction between G-actin and MKL proteins retains the coactivator within the cytoplasm of resting cells. Activated Rho-A promotes F-actin assembly and a reduction of the G-actin pool in serum-stimulated cells. This results in the accumulation of MKL proteins in the nucleus, where the coactivator associates with the SRF to activate target gene transcription and mediate multiple cellular processes (1-4). A number of other signaling pathways, including the TGFβ, BMP, and PDGF pathways, also make use of MKL-mediated activation of target gene transcription (5-9). Chromosomal translocations involving the genes encoding MKL1 and MKL2 have been identified in several cases of acute megakaryoblastic leukemia and chondroid lipoma (10-12). Alternate Names basic, SAP and coiled-coil domain; BSAC; KIAA1438; MAL; megakaryoblastic leukemia (translocation) 1; Megakaryoblastic leukemia 1 protein; Megakaryocytic acute leukemia protein; MKL; MKL/myocardin-like protein 1; MKL1; MRTF-A; MRTFA; myocardin related transcription factor A; Myocardin-related transcription factor A; RBM15/MKL1 fusion; RNA-binding motif protein 15/megakaryoblastic leukemia-1 fusion protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 145 Source/Isotype: Rabbit IgG