CST, 97118S, c-Maf (E2Q5D) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying MAF. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, c-Maf (E2Q5D) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #97118) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity c-Maf (E2Q5D) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human c-Maf protein. Background Transcription factor c-Maf, also known as MAF and V-maf musculoaponeurotic fibrosarcoma oncogene homolog, is a basic leucine zipper transcription factor (bZIP) in the large Maf protein subclass and belongs to the AP-1 superfamily (1). In humans, there are three c-Maf isoforms and in mice, only two isoforms have been characterized (2). Maf proteins contain a highly conserved ancillary DNA binding domain, N-terminal to the basic domain, and bind to relatively long DNA sequences termed Maf-recognition elements (MAREs) (3). c-Maf can form homodimers and heterodimers with other bZIP proteins like Jun and Fos, as well as non-bZIP proteins (4,5). c-Maf plays an important role in lens fiber cell development, bone development, apoptosis, and the immune system (6,7). In T cells, c-Maf expression is induced by TCR stimulation, and sustained by co-stimulatory signals, and/or cytokines like IL-4, IL-6, TGF-β, and IL-27 (8-12). In macrophages, c-Maf is required for self-renewal and promotes IL-10 production while inhibiting IL-12 (13,14). c-Maf plays a critical role in intestinal immune homeostasis, regulating ILC3s, Th17 cells, γδ T cells, and regulatory T (Treg) cells (7). c-Maf is an oncogene that contributes to the progression of some hematological malignancies and is expressed in other cancers but is not always correlated with poor prognosis (15,16). Alternate Names Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; AYGRP; c-MAF; c-maf proto-oncogene; CCA4; cMaf; CTRCT21; MAF; MAF bZIP transcription factor; MGC71685; Proto-oncogene c-Maf; T lymphocyte c-maf long form; Transcription factor Maf; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog; v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG