CST, 97174SF, NCAM1 (CD56) (123C3) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying NCAM1. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, NCAM1 (CD56) (123C3) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #97174) is ready to ship. Product Usage Information This product is the carrier free version of product #3576. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3576 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity NCAM1 (CD56) (123C3) Mouse Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total CD56 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing BALB/c mice with a membrane preparation of a small cell lung carcinoma specimen. Background NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Structural diversity is introduced by alternative splicing resulting in different cytoplasmic domains (1). NCAM mediates neuronal attachment, neurite extension, and cell-cell interactions through homo and heterophilic interactions. PSA (polysialic acid) post-translationally modifies NCAM and increases the metastatic potential of small cell lung carcinoma, Wilms' tumor, neuroblastoma, and rhabdomyosarcoma (2). CD56 is commonly used along with CD3 and CD16 to identify human natural killer (NK) cells (mouse NK cells do not express CD56) (3). Human NK cells are CD3-CD56+. The large subset with high CD16 expression are mature cytotoxic NK cells, while those with low CD16 expression are immature precursors and cytokine producers (4,5). Alternate Names antigen recognized by monoclonal antibody 5.1H11; CD56; MSK39; N-CAM-1; NCAM; NCAM-1; NCAM1; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120 to 220 Source/Isotype: Mouse IgG1