CST, 97189S, T-bet/TBX21 (E4I2K) Rabbit Monoclonal Antibody (Alexa Fluor® 555 Conjugate)

Monoclonal Antibody for studying TBX21 mouse. Validated for Immunofluorescence (Frozen),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, T-bet/TBX21 (E4I2K) Rabbit Monoclonal Antibody (Alexa Fluor^® 555 Conjugate) (CST #97189) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:100 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity T-bet/TBX21 (E4I2K) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment derived from mouse T-bet/TBX21 protein. Background The gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of the T-box family (7-9). Alternate Names OTTMUSP00000002150; T-bet; T-box 21; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Tbet; Tbly; Tblym; TBT1; Tbx21; Transcription factor TBLYM Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG