CST, 97206S, ABCD3 (F2N8W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ABCD3. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ABCD3 (F2N8W) Rabbit Monoclonal Antibody (CST #97206) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ABCD3 (F2N8W) Rabbit mAb recognizes endogenous levels of total ABCD3 protein. This antibody detects a 100 kDa and a 25 kDa protein of unknown identity in some cell lines. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ABCD3 protein. Background ATP-binding cassette sub-family D member 3 (ABCD3) is a member of the ABC transporter family, a group of evolutionarily conserved membrane proteins that use ATP hydrolysis to transport diverse substrates across biological membranes (1). ABCD3 was first cloned from rat liver peroxisomes as a 70 kDa peroxisomal membrane protein named PMP70 (2). At the peroxisomal membrane, ABCD3 utilizes a nucleotide-binding domain (NBD) and a transmembrane domain (TMD) to create a cavity that enables transport of its substrates, fatty acyl-CoAs, from the cytosol into the peroxisome for β-oxidation (3). Mutations in the gene have been linked to hepatosplenomegaly (4). Alternate Names 70 kDa peroxisomal membrane protein; ABC43; ABCD3; ATP binding cassette subfamily D member 3; ATP-binding cassette sub-family D member 3; ATP-binding cassette, sub-family D (ALD), member 3; CBAS5; dJ824O18.1 (ATP-binding cassette, sub-family D (ALD), member 3 (PMP70, PXMP1)); peroxisomal membrane protein 1 (70kD, Zellweger syndrome); Peroxisomal membrane protein-1 (70kD); PMP70; PXMP1; ZWS2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG