CST, 97298T, CCDC22 (E3F5L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CCDC22. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CCDC22 (E3F5L) Rabbit Monoclonal Antibody (CST #97298) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CCDC22 (E3F5L) Rabbit Monoclonal Antibody recognizes endogenous levels of total CCDC22 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CCDC22 protein. Background Coiled-coil domain-containing protein 22 (CCDC22) is a highly conserved protein that harbors an N-terminal calponin homology-like (NN-CH) domain and a C-terminal coiled-coil domain. CCDC22 is a core subunit of the COMMD/CCDC93/CCDC22 (CCC) protein complex, which also consists of CCDC93 and any one of ten COMMD proteins. The CCC complex, which is a subcomplex of the commander complex, plays a key role in protecting the stability and promoting the endosomal recycling of cell surface transmembrane proteins, such as integrins, LDLR, and the ATP7A copper transporter (1-3). Research studies have also demonstrated that as part of a ubiquitin ligase complex, CCDC22 activates proinflammatory NF-κB signaling by promoting the ubiquitin-dependent degradation of IκB proteins (4). Alternate Names CCD22; CCDC22; coiled-coil domain containing 22; Coiled-coil domain-containing protein 22; CXorf37; JM1; RTSC2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 72 Source/Isotype: Rabbit IgG