CST, 9731S, Cleaved DFF45 (Asp224) Antibody

Polyclonal Antibody for studying DFF45 (Asp224, C-term SS) cleaved. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cleaved DFF45 (Asp224) Antibody (CST #9731) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cleaved DFF45 (Asp224) Antibody detects the carboxy-terminal fragment of DFF45 resulting from cleavage at aspartic acid 224. This antibody does not recognize full length DFF45 or other cleavage products of DFF45. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp224 of human DFF45. Antibodies are purified by protein A and peptide affinity chromatography. Background Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspase-activated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the latter (1-4). In vitro, DFF45 has been shown to be the target of several caspases, including caspase-3, -6, -7, -8 and granzyme B (3). In vivo, caspase-3 is believed to be the primary enzyme responsible for processing DFF45 and release of its carboxy-terminal fragment (3,5). The cleavage of DFF45 inactivates its inhibitory function on DFF40 and causes nuclear DNA degradation by DFF40, leading to cell death (6,7). (This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.) Alternate Names DFF-45; DFF1; DFF45; DFFA; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; Inhibitor of CAD Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 12 SOURCE: Rabbit