CST, 9732S, DFF45/DFF35 Antibody

Polyclonal Antibody for studying DFF45. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, DFF45/DFF35 Antibody (CST #9732) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DFF45/DFF35 Antibody detects endogenous levels of total full length DFF45 and/or full length DFF35. This antibody does not cross-react with related proteins. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp224 of human DFF45. Antibodies are purified by protein A and peptide affinity chromatography. Background Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspase-activated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the latter (1-4). In vitro, DFF45 has been shown to be the target of several caspases, including caspase-3, -6, -7, -8 and granzyme B (3). In vivo, caspase-3 is believed to be the primary enzyme responsible for processing DFF45 and release of its carboxy-terminal fragment (3,5). The cleavage of DFF45 inactivates its inhibitory function on DFF40 and causes nuclear DNA degradation by DFF40, leading to cell death (6,7). Alternate Names DFF-45; DFF1; DFF45; DFFA; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; Inhibitor of CAD Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35, 45 SOURCE: Rabbit