CST, 9737S, Phospho-MEF2A (Ser408) Antibody

Polyclonal Antibody for studying MEF2A (Ser408) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-MEF2A (Ser408) Antibody (CST #9737) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-MEF2A (Ser408) Antibody detects transfected levels of MEF2A only when phosphorylated at Ser408. This antibody has also been shown to detect phospho-MEF2A (Ser408) in granule neurons (see application reference). Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser408 of human MEF2A. Antibodies are purified by protein A and peptide affinity chromatography. Background MEF2A is a member of the MEF2 (myocyte enhancer factor 2) family of transcription factors. In mammals, four MEF2A-related genes (MEF2A, MEF2B, MEF2C and MEF2D) encode proteins which exhibit significant amino acid sequence similarity within their DNA binding domains and to a lesser extent throughout the remaining proteins (1). The MEF2 family members were originally described as muscle-specific DNA binding proteins that recognize MEF2 motifs found within the promoters of many muscle-specific genes (2,3). Phosphorylation of MEF2A at Thr312 and Thr319 within the transcription activation domain by p38 MAP kinase enhances MEF2A-MEF2D heterodimer-dependent gene expression (4). On the other hand, apoptotic stimuli (e.g. neurotoxic insult) result in CDK5-dependent phosphorylation of MEF2A at Ser408 within the activation domain, inhibiting MEF2A pro-survival function (5). Alternate Names ADCAD1; MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A); MEF2; MEF2A; myocyte enhancer factor 2A; Myocyte-specific enhancer factor 2A; RSRFC4; RSRFC9; Serum response factor-like protein 1 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 54 SOURCE: Rabbit