CST, 97585S, CD11c (D1V9Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ITGAX mouse. Validated for WB,IHC,IHC,IF,IF. Available in 3 sizes. Highly specific and rigorously validated in-house, CD11c (D1V9Y) Rabbit Monoclonal Antibody (CST #97585) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:150 - 1:600 Immunohistochemistry (Paraffin): 1:150 - 1:600 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 39143 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CD11c (D1V9Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD11c protein. Non-specific staining of the epithelium in fixed frozen mouse intestine was observed by immunofluorescence. Non-specific staining in fixed frozen mouse brain was observed by immunofluorescence. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1153 of mouse CD11c protein. Background CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7). Alternate Names AI449405; CD11 antigen-like family member C; Cd11c; CD11C (p150) alpha polypeptide; complement receptor 4; Cr4; integrin alpha X; Integrin alpha-X; integrin aX; ITAX; Itgax; leukocyte adhesion glycoprotein p150,95 alpha chain; leukocyte adhesion receptor p150,95; mFLJ00114; MFLJ00114 protein; N418 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 145 Source/Isotype: Rabbit IgG